Plant endophytic bacteria (endophytes) are found in most plant species. Research on endophyte bacteria shows that they help promote plant growth, increase productivity and act as a biological regulating agent. In addition, they have the potential to remove soil pollutants by enhancing plants' detoxification capabilities, making the soil fertile. Currently, many scientists are interested in developing biotechnology applications of endophyte bacteria to develop plant varieties capable of detoxification, and at the same time capable of producing biomass and biofuel.
In recent years, nanotechnology has become an attractive research field and is widely used in the biomedical and agricultural industries. In agriculture, crop yields are significantly improved through the use of essential micronutrients in the form of nano fertilizers. Silica exists in abundance in the soil and is absorbed from the soil through the roots of the plant and remains in the trunk of the plant. Many studies have shown that silica nanoparticles are very important in developing resistance in plants. Silica is considered an essential micronutrient and is known for its role in enhancing plant growth. The application of silica to plants has been documented in many previous studies.
Studies of integrating nanomaterials with endophyte bacteria have emerged recently and only a handful of reports have been published. Therefore, this thesis investigates the effect of the integration of endophyte bacteria (Bacillus subtilis GB03) with nano silica produced by sol-gel method combined with ultrasound for the purpose of developing products of natural origin, Safe when using.
Nano silica with size 20-50 nm, fabricated by ultrasonic sol-gel method, is used to test the effect of nano silica on the growth of Bacillus subtilis GB03 in the room. experiment. At the same time, evaluate the effect of nano silica integrated bacteria B. subtilis GB03 on the growth of melon plant (Cucumis melo). The results showed that nano silica at the concentration of 10 µg / ml had a positive effect on the growth of the bacteria B. subtilis GB03. In the in vivo experiment, the team observed the phenomenon of SiO2 nanoparticles attaching to the bacterial surface and the bacterial penetration into the roots. At the same time, the integration of bacteria and nano silica at a concentration of 10 µg / ml also showed a good effect on the germination rate and rate, root development, growth time, height and ability. branching function of melon plant. Research opens a direction to develop natural endophyte nano-silica-microbial combination products to replace chemical drugs to develop melon plants in particular and plants in general.
nthang - Trithuckhoahoc, according to Vietnam S&T Corporation
